Two Arabidopsis thaliana shikimate kinases, AtSK1 and AtSK2, were previously identified as having arisen from a duplication event 40-60 million years ago and are both believed to function in the shikimate pathway. We investigated the homologs have acquired divergent regulatory or functional roles since duplication. AtSK1 demonstrates different transcript levels than AtSK2 during heat shock and in floral tissues as determined by RT-PCR. Mining of publically available microarray datasets identified HSF1 and MYB family transcription factors as possible regulators of AtSK1 under these conditions. Heat shock response did not appear to be affected by either sk1 or sk2 knockouts in assays measuring thermotolerance and ROS production. Floral morphology appears normal during floral stages corresponding with AtSK1 transcript induction however, pollen viability is reduced by 20% in sk1 knockouts as measured by FDA staining. Thus, this work has identified instances of differential regulation and function between two recently duplicated shikimate kinases.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:OTU.1807/65542 |
| Date | 24 June 2014 |
| Creators | Buranyi, Stephen |
| Contributors | Christendat, Dinesh |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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