Traditional screens for female sterile mutants have revealed only two genes which when mutant, give a fully penetrant 16 nurse cell phenotype. One way to gain a better understanding of the function of these genes in oocyte determination, is to identify genes which interact with them. Using P-element mutagenesis, I have isolated one dominant suppressor and five dominant enhancers of Bicaudal-D, and begun the phenotypic and molecular characterization of three of these genes. By deficiency screening, I have identified two different loci which act as dominant enhancers of Bic-D, and eight different loci which act as dominant suppressors of Bic-D. Further work on defining the locus responsible for the strong suppression phenotype associated with one of these deficiencies, revealed betaH-spectrin to be a strong dominant suppressor of loss-of-function Bic-D alleles, and a strong dominant enhancer of Bic-D gain-of-function alleles.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20598 |
| Date | January 1997 |
| Creators | Nguyen, Thuy, 1973- |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001609628, proquestno: MQ44232, Theses scanned by UMI/ProQuest. |
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