Our ongoing hypothesis is that skeletal muscle satellite cells can be injected into injured myocardium with the intent of myocardial repair. In early experiments tritiated thymidine was used to label the satellite cells prior to injection into the injured myocardium. Tritiated thymidine however was not seen in the retrieved heart specimens. This gave rise to the criticism that there was no definitive proof that cardiac tissue fibres originating in the injured myocardium were a result of the satellite cell implantation. It was felt that tritiated thymidine was an inadequate label possibly due to a dilutional effect. It was also thought that the cryo-injury was too severe to allow for a sufficient micro environment for cell growth. The purpose of the present study was to develop an appropriate labelling technique that would confirm without doubt that the satellite cells injected indeed led to myocardial regeneration. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.20957 |
| Date | January 1998 |
| Creators | Greentree, David. |
| Contributors | Chiu, R. C. J. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001630784, proquestno: MQ50779, Theses scanned by UMI/ProQuest. |
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