The optimization of isolation and characterization of chitin deacetylase (CDa) from Mucor rouxii was the focus of this study. The crude extract in the active form was partially purified by ammonium sulfate fractionation, followed by column chromatography by ion exchange in the Fast Protein Liquid Chromatography (FPLC) system. / Using a 10 mM concentration, the order of effectiveness for the inhibitors in achieving 35--40% was CaCl2, CuSO4, MgCl 2 and EDTA. At a 25 mM concentration, propionic acid and sodium acetate inhibited the enzyme up to 40% & 50%, respectively. M. rouxii CDa activity was greater in the mycelial extract, and was capable of efficiently hydrolyzing the substrate glycol chitin. / Two major bands were observed after native PAGE of the partially purified enzyme using Mono Q column (FPLC). The estimated molecular weight of CDa bands by SDS-PAGE containing 0.1% glycol chitin was 21, 23, 26 and 44 kDa when compared to the migration of molecular weight markers. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.21548 |
| Date | January 1999 |
| Creators | Eltaib, Farag Ibrahim. |
| Contributors | Simpson, B. K. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Food Science and Agricultural Chemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001657768, proquestno: MQ50763, Theses scanned by UMI/ProQuest. |
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