This thesis reports on some sequencing and functional studies on chromosome I of Saccharomyces cerevisiae. / Eight open reading frames (ORFs) have been identified in S. cerevisiae which have similarity to the canine 24kD glycoprotein, gp25L (Wada et al., 1991). In chapter 2 of this thesis, I report the gene disruption and functional characterization of three of these ORFs: YAR002Ac, YAL007c and YGL002w. Disruption of YAR002Ac resulted in calcofluor white resistance, disruption of YGL002w increased sensitivity to this compound, while yal007c$ Delta$ mutants had no calcofluor white phenotype. The expression of Kre9p was partially increased in the ygl002w$ Delta$ mutant. All single, double and triple mutants grew, mated and sporulated normally. / The sequence of each S. cerevisiae chromosome, released in GenBank database, lacked most of the telomere sequence. This was due to the fact that a telomeric fragment with one clonable end cannot be integrated into a vector by the classical cloning method. Recently, Louis and Borts cloned all telomeres of S. cerevisiae successfully using an alternative cloning method (Louis and Borts, 1995). In chapter 3 of this thesis, I report the DNA sequence of the right telomeric region of chromosome I. The sequence indicates that this region represents a typical yeast telomeric region and contains 98bp of TG$ sb{1-3}$ repeats and an X subtelomeric element. Another subtelomeric element, the Y$ sp prime$ element, is absent from the right telomeric region of chromosome I. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27439 |
| Date | January 1996 |
| Creators | Zhong, Wuwei. |
| Contributors | Bessey, Howard (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001578380, proquestno: MQ29814, Theses scanned by UMI/ProQuest. |
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