Injured adult mammalian cardiac muscle does not respond with significant regeneration. Skeletal muscle, on the other hand, has an efficient regeneration mechanism attributable to myogenic stem (satellite) cells. This study investigated a hypothesis which states that skeletal muscle satellite cells (SCs), if grafted into an injured heart, can form muscle tissue and repair the damaged myocardium. / In the first study SCs obtained from 28 dogs were isolated, purified and cultured. Percoll density gradient and preplating methods for the purification of SC culture were investigated. Beta-galactosidase ($ beta$-Gal) labelled SCs underwent autografting into acutely cryoinjured myocardium. Three control dogs received only sham implants into the cryoinjured heart. Two (n = 6), 4 (n = 16) and 6 (n = 6) weeks after SC implantation, hearts with SC graft sites were procured and processed for histrionical examination, as well as for detection of a $ beta$-Gal marker. Control specimens were obtained at 2 (n = l), 4 (n = l), and 6 (n = l) weeks after sham implantation and were processed as described above. / In the second study on rats, SCs were obtained from donor animals (n = 30). An intramuscular bupivicaine method to increase SC yield was adopted. Purified, cultured, and $ beta$-Gal labelled SCs were implanted either into acutely cryoinjured myocardium (n = 15), or into the mature cardiac scar (n = 15) of isogenic donor animals. Myocardial specimens were obtained at 1 month after SC implantation. Techniques of rodent SC labelling in vitro with human alkaline phosphorate genes were also explored. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27440 |
| Date | January 1997 |
| Creators | Zibaitis, Audrius. |
| Contributors | Chiu, Ray C.-J. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001565158, proquestno: MQ29815, Theses scanned by UMI/ProQuest. |
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