A lesion interface consisting of a glia limitans lined by a laminin-rich basal lamina forms within 3 weeks after leptomeningeal cells infiltrate into penetrating wounds in the adult mammalian CNS. This impedes axon regrowth. We have examined the effects of steroids on the formation of the lesion interface in the adult rat cerebral cortex. Topical glucocorticoids were applied on the surface of cortex encompassing stab wounds. Three weeks later, cryostat sections through the lesioned area were labeled with anti-laminin, anti-GFAP, anti-ED-1 and Nuclear Yellow. Steroid treatment attenuated all components of the lesion, including leptomeningeal cell infiltration. In vitro, steroid treatment did not alter laminin secretion but reduced cell proliferation in leptomeningeal cultures. These results suggest that steroids modulate lesion interface formation in the CNS in part by decreasing leptomeningeal cell proliferation.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.27541 |
| Date | January 1997 |
| Creators | Li, Maria S. |
| Contributors | David, Samuel (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Neurology and Neurosurgery.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001601801, proquestno: MQ37140, Theses scanned by UMI/ProQuest. |
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