Vesicle (exosome) externalization is a mechanism for removal of obsolescent membrane proteins, such as the transferrin receptor (TFR), during reticulocyte maturation. This thesis involves further characterization of the exosomal phenomenon using avian (nucleated) and mammalian erythroid systems. Chicken reticulocytes release exosomes, indicating independence of this process from enucleation. Exosome release during chicken erythroleukemic cell (HD3) differentiation (representing pre-reticulocyte development) demonstrates that this process commences prior to the reticulocyte stage. / The heat shock protein, hsp70, has been found in exosomes from every species examined (four species, including the chicken). It is shown that hsp70 is physically, but non-covalently, associated with exosomal TFR, suggesting its possible involvement in targeting proteins into exosomes during their formation. / Exosome formation is an energy-dependent process. Our observations indicate that the primary energy substrate for avian red cells is not glucose, but glutamine, inosine and guanosine. Work with the HD3 cells (capable of glucose transport), suggests there is an early differentiation-associated switch in the chicken red cell's ability to use glucose as a major energy substrate.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.28838 |
| Date | January 1994 |
| Creators | Mathew, Anu |
| Contributors | Johnstone, R. M. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biochemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001441132, proquestno: NN05752, Theses scanned by UMI/ProQuest. |
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