Secondary peritonitis is a significant cause of morbidity and mortality in the ICU and ICU patients as a group have the highest rate of nosocomial infections. Once recruited to the site of injury, the PMN interacts with endothelial cells (ECs) via rolling adhesion, firm adhesion, and transendothelial migration. Using a murine cecal ligation and puncture peritonitis model and either skin or cremaster injury as the secondary site in a two-front injury model, we examined the role of injury severity on the triage of PMNs to competing sites of injury. We demonstrated that a finite pool of PMNs was recruited to tissues in numbers correlating to the severity major injury. With intravital microscopy we demonstrated that numbers of PMNs involved in rolling adhesion, rolling velocity and stationary adhesion in the presence of one or more sites of injury were predictable, consistent and likely mediated by changes in surface adhesion molecule expression.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.29924 |
| Date | January 1999 |
| Creators | Swartz, Daniel E. |
| Contributors | Christou, Nicolas V. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001681849, proquestno: MQ55091, Theses scanned by UMI/ProQuest. |
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