The Mpt5 protein of Saccharomyces cerevisiae has been implicated in a variety of cellular processes including cell cycle regulation, aging, cell wall maintenance and regulation of gene transcription by glucose. Loss of Mpt5p results in temperature-sensitive cell lysis which is osmotically remedial. In this thesis, synthetic lethalities of the mpt5Delta msn5Delta double mutant and the cln1Delta cln2Deltampt5Delta triple mutant are reported. A multicopy suppressor screen identified amino-terminal truncations of Mpt5p and the HMLalpha silent mating type locus as dosage-dependent suppressors of the mpt5Delta is lethality. Differential gene expression analysis in mpt5Delta revealed that several genes which code for cell wall structural proteins and genes which are under control of the Tup1p-repressor are less expressed in the absence of Mpt5p. Finally, Green Fluorescent Protein-tagged Mpt5p has allowed for investigation of Mpt5p cellular localization.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.30746 |
| Date | January 1999 |
| Creators | Sherk, Jennifer. |
| Contributors | Whiteway, Malcolm (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001746675, proquestno: MQ64451, Theses scanned by UMI/ProQuest. |
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