The objectives of this study were to determine the effect of different types and levels of dietary fiber on Heligmosomoides polygrus (Nematoda) in laboratory mice. BALB/c mice were randomly assigned to one of seven diets: no fiber, cellulose (5%, 10%, 20%), and pectin (5%, 10%, 20) and infected with H. polygyrus one week later. The length of intestine increased significantly with the concentration of pectin and was associated with longer villi, thicker mucosa and increased villus/crypt ratio. In contrast, changing concentration of cellulose had minimal effects on the gastrointestinal tract. More day 5 larvae were found in mice fed 20% pectin, but neither type nor level of dietary fiber affected worm survival or spatial distribution. An increase from 5% to 10% pectin increased per capita egg production both in vivo and in vitro, but a further increase to 20% pectin decreased per capita egg production in vivo to the low level seen in mice fed no fiber. However when the parasites from mice fed 20% pectin diet were cultured in vitro, they released very high numbers of eggs. Changing concentration of cellulose had no effect on parasite egg production in vivo or in vitro. Our study supports the hypothesis that pectin promotes the establishment and reproduction of Heligmosomoides polygyrus, and that this is closely related to the changes in gut morphology. Therefore we conclude that cellulose, not pectin, should be used to balance reduced content of macronutrients in studies on malnutrition and nematode infection.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.31547 |
| Date | January 2001 |
| Creators | Sun, Yi, 1973- |
| Contributors | Scott, M. E. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Institute of Parasitology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001810370, proquestno: MQ70511, Theses scanned by UMI/ProQuest. |
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