Distraction osteogenesis is a form of in vivo tissue engineering used to lengthen bone. Bone morphogenetic proteins (BMPs) are known mediators of bone formation during the distraction of enchondral bones. Their role in the distraction of membranous bones remains unknown. A rabbit model was used to characterize the expression of BMP-2, 4 and 7 during mandibular distraction osteogenesis. Fourteen animals were subjected to a defined distraction protocol. At weekly intervals, two rabbits were sacrificed and the generate bone harvested for radiographic, bone densitometric, histologic and immunohistochemical analyses. The results demonstrate that during distraction, bane forms primarily by intramembranous ossification. BMP-2 and 4 were maximally expressed in osteoblastic cells during the distraction period and in chondrocytes during the consolidation period. The pattern of mandibular BMP expression was different than previously described for long bones. Understanding the pattern of BMP expression may guide the strategic administration of recombinant BMPs to enhance the rate and quality of bone formation during distraction osteogenesis thereby shortening the time required for consolidation of the generate bone.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.32980 |
| Date | January 2001 |
| Creators | Campisi, Paolo. |
| Contributors | Lessard, M. L. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Otolaryngology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001838386, proquestno: MQ75290, Theses scanned by UMI/ProQuest. |
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