Limb salvage in patients with advanced peripheral vascular disease or effective nonoperative therapy for patients with limited disease still remains a challenge in modern vascular surgery. Based on recent advances in the area of angiogenesis as well as our previous studies of alternative approaches for limb ischemia, we have presented herein the step-by-step experimental investigations that are directed toward the development of a novel therapy, angiogenic stimulation, for the revascularization of ischemic limbs. An animal ischemic hindlimb model has been developed suitable for testing our hypothesis and further understanding of this potential therapy. We have demonstrated that an angiogenic factor, ECGF, when administered intramuscularly into the ischemic limb, markedly enhances revascularization of the limb in the models of unilateral as well as bilateral hindlimb ischemia. In addition, we have also demonstrated that the angiogenic effect of ECGF is dose dependent and is demonstrable only when it is administered directly into the limb in the presence of ischemia. Therefore, we conclude that angiogenic therapy may have the potential for the treatment of patients with chronic limb ischemia.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.41308 |
| Date | January 1993 |
| Creators | Pu, Li-Qun |
| Contributors | Symes, James F. (advisor), Sniderman, Allan D. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001351891, proquestno: NN91858, Theses scanned by UMI/ProQuest. |
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