The combination of pretransplant donor specific transfusion (DST) and cyclosporin (Cys) has proven to be an effective mode of immunomodulation in numerous allograft models. Our experiments were designed to study the effect of clinically applicable protocols using DST and low-dose cyclosporin in an heterotopic, fully allogenic model of small bowel transplantation in the rat. / A 1 ml systemic DST 24 hours pretransplant with Cys (10 mg/kg day $-$1, 5 mg/kg POD 0 to 7, 2.5 mg/kg POD 8 to 14) was shown to be more effective than DST or Cys alone in prolonging graft survival (p $<$ 0.05). Adding successive post-transplant DST (POD 7,14,21) had no effect on graft survival. Portal transfusion and Cys was the most effective mode of antigen presentation (p = 0.01 vs systemic DST), with 33% of the animals having prolonged survival. Adding successive post-transplant DST was deleterious to the portal DST effect. The adjunct of anti-lymphocyte serum to the DST-Cys combination was ineffective.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.56981 |
| Date | January 1992 |
| Creators | Fecteau, Annie |
| Contributors | Guttman, Frank M. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001319313, proquestno: AAIMM87722, Theses scanned by UMI/ProQuest. |
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