Fibroblasts from patients with functional methionine synthase deficiency can be divided into 2 complementation classes, cblE and cblG. Both have low levels of intracellular methylcobalamin. Both groups also demonstrate low levels of incorporation of label from 5-methyltetrahydrofolate into macromolecules. Under standard reducing conditions, methionine synthase specific activity is normal in cblE fibroblast extracts, but is low in cblG fibroblast extracts. Seven cblE and seven out of ten cblG cell lines demonstrate levels of accumulation of ($ sp{57}$Co) CN-Cbl in fibroblasts comparable to that of control cells. They exhibit similar proportions of label associated with the two intracellular cobalamin binders, methylmalonyl-CoA mutase and methionine synthase. The remaining three cblG cell lines exhibit a lower level of cobalamin accumulation, and demonstrate a lack of cobalamin association with the enzyme methionine synthase. The specific activity of methionine synthase is almost undetectable in the three cblG cell lines that showed no such association. These results demonstrate heterogeneity within the cblG group and suggest that the defect in cblG affects the methionine synthase apoenzyme.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.60091 |
| Date | January 1991 |
| Creators | Sillaots, Susan L. (Susan Linda) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001237965, proquestno: AAIMM67831, Theses scanned by UMI/ProQuest. |
Page generated in 0.0026 seconds