This thesis reviewed current theories of the post-ischemic injury. Special emphasis was placed on the concept of reperfusion injury. / In a series of four experiments, this thesis evaluated neutrophil function, localization, depletion, and inhibition in ischemic cutaneous and myocutaneous flaps. Neutrophil function as measured by phorbol myristate acetate stimulated superoxide production was found to be enhanced on reperfusion of ischemic myocutaneous flaps. $ sp{111}$Indium labelled neutrophils were found to localize in post-ischemic cutaneous and myocutaneous flaps. Post-ischemic cutaneous flap survival was not improved by either cyclophosphamide induced neutrophil depletion or ibuprofen neutrophil inhibition. Post-ischemic myocutaneous flap survival was significantly improved by both cyclophosphamide induced neutrophil depletion and ibuprofen neutrophil inhibition. Thus, the cumulative evidence from these experiments yielded surprisingly different conclusions for cutaneous and myocutaneous flaps. The post-ischemic injury in cutaneous flaps was not mediated by the neutrophil. In contrast, the neutrophil did partially mediate the complex post-ischemic injury of myocutaneous flaps.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.60431 |
| Date | January 1990 |
| Creators | Lee, Chen |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001213383, proquestno: AAIMM67567, Theses scanned by UMI/ProQuest. |
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