Return to search

The metabolism of tRNAAspargine in the friend erthroleukemia cell /

I have measured the metabolism of tRNA$ sp{ rm Asn}$ in Friend cells undergoing erythroid differentiation, using an assay exploiting the tRNA$ sp{ rm Asn}$ gene's ability to specifically hybridize tRNA$ sp{ rm Asn}$. The concentrations of tRNA$ sp{ rm Asn}$ measured as a proportion of the total tRNA population (relative concentration) decreased until day 3 post-induction, and then increased on day 4. The relative concentrations of tRNA$ sp{ rm Asn}$ are influenced by tRNA$ sp{ rm Asn}$ having lower turnover rate in comparison to the total tRNA population, and by the relative rate that labelled, newly synthesized tRNA$ sp{ rm Asn}$ accumulates in vivo. The rate of tRNA$ sp{ rm Asn}$ synthesis in nuclei isolated from Friend cells at different times during differentiation also fluctuates. This fluctuation may reflect changes in RNA polymerase III activity in these isolated nuclei.

Identiferoai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.60725
Date January 1992
CreatorsMiller, Harvey
PublisherMcGill University
Source SetsLibrary and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada
LanguageEnglish
Detected LanguageEnglish
TypeElectronic Thesis or Dissertation
Formatapplication/pdf
CoverageMaster of Science (Department of Biology.)
RightsAll items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated.
Relationalephsysno: 001287765, proquestno: AAIMM74587, Theses scanned by UMI/ProQuest.

Page generated in 0.0021 seconds