The effects of ischemia/reperfusion injury on humoral changes in canines that underwent left lung allotransplantation was compared to autotransplantation (n = 10 each). Cytokines IL-2, TNF-a and IFN-g were measured in bronchoalveolar lavage (BAL) and plasma samples at 1, 4, 24 hours and 1 week postoperatively. In the allograft there was an early postoperative increase in all cytokines in BAL which decreased after 24 hours. The same trend was seen for IL-2 in the autograft. In contrast, TNF-a and IFN-g levels in the autograft remained unchanged. Using immunohistochemical staining techniques, MHC II antigen expression was observed on lung allograft bronchial epithelium which was less intense in the autograft. Hematoxylin and eosin staining of lung biopsies revealed early evidence of lung injury and also grade 1-2 rejection after 1 week in the allograft. Injury was not as severe in the autograft. We conclude that a temporary elevation of cytokines early after allotransplantation is partly due to ischemia/reperfusion injury and graft allogenicity. This early cytokine release may play an important role in the development of early graft dysfunction and rejection.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69540 |
| Date | January 1992 |
| Creators | Serrick, Cyril James |
| Contributors | Shennib, Hani (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Division of Surgical Research.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001323921, proquestno: AAIMM87838, Theses scanned by UMI/ProQuest. |
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