Light microscopic immunoperoxidase staining of rat testes with polyclonal anti-Sm and monoclonal anti-Y$ sb{12}$ localized U1, U2, U4/U6 and U5 (spliceosome) snRNPs predominantly to nuclei of germ cells up to step 10 spermatids. The absence of reactivity after step 11 is concomitant with nuclear condensation and transcriptional arrest (Monesi, 1964). Nuclear and cytoplasmic reactivity in pachytene spermatocytes reached a peak at stage XII, coincident with maximal rates of RNA synthesis (Monesi, 1964). Quantitative EM immunogold labeling of Lowicryl embedded testicular sections confirmed the light microscope observations. Immunogold speckles were observed along nuclear chromatin. The chromatoid bodies of spermatids and spermatocytes and the intermitochondrial material of spermatocytes were found to be additional sites of snRNP localization. This co-localization suggests that these dense cytoplasmic structures are related. Anti-U1 snRNP antibodies applied to frozen sections co-localized with spliceosome snRNPs. Anti-U3 snRNP antibodies applied to frozen sections stained nucleoli of germ cells where pre-rRNA is usually spliced.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.69753 |
| Date | January 1993 |
| Creators | Moussa, Fuad |
| Contributors | Hermo, L. (advisor), Oko, R. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Anatomy and Cell Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001351839, proquestno: AAIMM91870, Theses scanned by UMI/ProQuest. |
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