A bacterium and a fungus identified as a Pseudomonas sp. and a Penicillium sp. were isolated by the soil perfusion enrichment technique, and are able to utilize phloroglucinol as the sole carbon source in a medium containing various inorganic salts. Chemical and physical factors affecting growth of the organism and the rate of phloroglucinol utilization were studied. A new pathway for the decomposition of phloroglucinol by the Pseudomonas sp. has been proposed. This pathway involves the removal of a hydroxyl group from phloroglucinol forming a conjugated phenolic compound followed by the oxidation of the bond between the third and fourth carbon atoms forming beta-ketoadipic acid. The enzymes involved in this pathway are not constitutive, but are induced through the formation of adaptive enzymes. The enzymatic reactions were NADPH2 dependent. The Km of the enzyme for phloroglucinol was determined. The Penicillium sp. utilizes phloroglucinol and accumulates a product which is not further metabolized.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.70087 |
| Date | January 1965 |
| Creators | Robern, Haskel. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Microbiology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000593843, proquestno: AAINK00319, Theses scanned by UMI/ProQuest. |
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