NAD-Dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is a mitochondrial folate-dependent enzyme apparently expressed only in transformed mammalian cells and tissues containing undifferentiated cells. The cloning of both the cDNA and the gene encoding this bifunctional enzyme have led to a better understanding of the regulation of its expression in normal and transformed cells. Southern blot analyses revealed that the gene has a similar organization in normal and transformed cells and no major rearrangement or amplification were detected. The gene encodes two messenger RNAs which differ only by the length of their 3$ sp prime$-untranslated region and are derived by the use of alternative polyadenylation signals. The detection of transcripts in transformed cells and not in normal adult tissues suggests that the gene is primarily regulated at the transcriptional level. The promoter region of the gene contains several putative cis-regulatory elements which may have an important role in the differential expression of this folate-dependent enzyme.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.70166 |
| Date | January 1990 |
| Creators | Bélanger, Carole |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Biochemistry.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001214713, proquestno: AAINN67513, Theses scanned by UMI/ProQuest. |
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