Lipid systems containing charged species are examined by both experiment and theory. Experimental studies of the mixing of phosphatidylcholine or phosphatidylethanolamine with phosphatidic acid show that calcium induces fast ($ leq$1s) phase separation of these otherwise miscible systems, and that this can occur in an isolated bilayer. Ionogenic behaviour is theoretically investigated using a new electrolyte model which explicitly includes both the solvent and particle sizes, and a binding model which uses Guggenheim combinatorics to treat non 1-1 binding stoichiometries. This work predicts a reduced dielectric constant near charged surfaces and strong repulsive forces between closely spaced ($<$15A) surfaces. A reanalysis of data from charged monolayers experiments indicates (1) that the new electrolyte model describes double layer behaviour at high surface charge densities better than the traditional Derjaguin - Landau - Verwey - Overbeek (DLVO) theory, (2) that calcium and magnesium bind to phosphatidylserine monolayers with a 1-1 stoichiometry.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.75664 |
| Date | January 1987 |
| Creators | Graham, Ian Stanley. |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Doctor of Philosophy (Department of Physics.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 000550044, proquestno: AAINL44484, Theses scanned by UMI/ProQuest. |
Page generated in 0.0018 seconds