Listeria monocytogenes is a human and animal pathogen responsible for severe and sometimes fatal infections. Several outbreaks have been associated with contaminated commercial foodstuffs such as raw milk, soft cheese, fresh and frozen milk, poultry, seafood, fruits and vegetable products. Currently, the official method recognized by the Government of Canada for the detection and isolation of L. monocytogenes can take up to six days without confirmation, which can require two more days. An approach based on molecular beacons that fluoresce upon hybridization was developed and tested to detect L. monocytogenes and the genus Listeria in food. Two different beacons were created: one specific to species L. monocytogenes (MG1) and another for the genus Listeria (MG2). Each of these molecular beacons was used with two separate sets of primers: MG1-1f/MG1-2r, MG1-7f/MG1-4r, MG2-2f/MG2-2r and MG2-3ft/MG2-2r. (Abstract shortened by UMI.)
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.78371 |
| Date | January 2002 |
| Creators | Groulx, Marylène |
| Contributors | Fortin, M. G. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Plant Science.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001985502, proquestno: AAIMQ88206, Theses scanned by UMI/ProQuest. |
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