Type II transmembrane serine proteases (TTSPs), including hepsin and corin, are a new class of cell surface catalytic enzymes. In the present study, a non-transmembrane isoform of hepsin, named hepsin/-TM that originates from alternative splicing, was identified. Unlike the transmembrane hepsin isoform, this non-transmembrane isoform was distributed within the cytoplasm and likely to be modified after translation. Real-time PCR experiments revealed that hepsin was expressed in all tested human tissues, but hepsin/-TM only in kidney, brain and lung tissues. Significantly, hepsin/-TM was not expressed in liver where hepsin was originally identified. However, hepsin/-TM was highly expressed in brain where hepsin was expressed at a significantly lower level. Moreover, these two isoforms showed different expression patterns in several colon cancer cell lines. Furthermore, ten corin-interacting proteins were identified and a variety of corin mutants were generated for the studies of the relationship of corin structure and function.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.79034 |
| Date | January 2003 |
| Creators | Li, Yang, 1974- |
| Contributors | Zhao, Xin (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Animal Science.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 001985480, proquestno: AAIMQ88249, Theses scanned by UMI/ProQuest. |
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