The efficient processing of replication-dependent histone mRNA requires the Stem-Loop Binding Protein (SLBP). SLBP is also involved in regulating histone mRNA half-life, their nucleocytoplasmic transport, and their translation. Unlike somatic cells, where SLBP protein accumulates only in S-phase, SLBP protein is present throughout the first two embryonic cell cycles in mice. We report here that in late 2-cell mouse embryos there is a substantial, proteasome-dependent decrease in SLBP throughout the cell. Based on chromosome morphology, the degradation of SLBP protein in late 2-cell embryos is most likely a late G2-phase event. The degradation of SLBP protein is not simply a zygotic clock event, but requires development to the late 2-cell stage. Furthermore, SLBP protein degradation in 2-cell mouse embryos requires cyclin-dependent kinase (Cdk) activity, DNA replication, and zygotic genome activation. A model for SLBP protein degradation is proposed based on observations made in both early mouse embryos and somatic cells.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.80351 |
| Date | January 2003 |
| Creators | Poirier, Luc |
| Contributors | Clarke, Hugh J. (advisor) |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Department of Biology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002084285, proquestno: AAIMQ98720, Theses scanned by UMI/ProQuest. |
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