Here we report the expression and purification of two recombinant Fasciola hepatica enzymes, catL2 and catL5 which were used to perform cleavage studies with substrates potentially encountered by the parasite in vivo; BSA, hIgG3K and the important T cell marker, CD4. We examined the digestion products generated by the cleavage of human CD4 with catL5 using mass spectrometry and predicted candidate cleavage sites by performing a theoretical digest of the protein. / Ovine CD4 is also of interest to us as a target of F. hepatica cathepsin L activity. Here we confirm a recently reported ovine CD4 cDNA sequence and the existence of a single nucleotide polymorphism (T/C) within this sequence. The polymorphism translates to a serine-proline switch near the hinge region of the protein. Additionally, we have found that this polymorphism is also present in genomic DNA, suggesting that two alleles of CD4 exist in the ovine genome.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:QMM.81601 |
| Date | January 2004 |
| Creators | Boscariol, Rya |
| Publisher | McGill University |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
| Format | application/pdf |
| Coverage | Master of Science (Institute of Parasitology.) |
| Rights | All items in eScholarship@McGill are protected by copyright with all rights reserved unless otherwise indicated. |
| Relation | alephsysno: 002211387, proquestno: AAIMR12404, Theses scanned by UMI/ProQuest. |
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