An isolate of <i>Colletotrichum truncatum</i> (Schwein.) Andrus & W. D. Moore is a promising bioherbicide candidate against scentless chamomile (<i>Matricaria perforata</i> Mérat), a noxious weed in western Canada. A major constraint in the development of this bioherbicide is the inefficiency of inoculum production. The objective of this study was to explore submerged fermentation for mass production of <i>C. truncatum</i>.<p>A defined basal salts medium (DBSM) was used for liquid culture with glucose and casamino acids selected as the optimal carbon (C) and nitrogen (N) sources, respectively. Spore yield and biomass production were significantly higher when the DBSM glucose concentration was 35-40 g/L compared to lower concentrations, while inoculum efficacy was significantly greater when produced at 5-10 g/L than at 40 g/L of glucose. Spore yield in baffled flasks at 200 RPM shaker speed was significantly higher than in regular flasks at lower shaker speeds. Under conditions of high aeration, glucose concentration had a significant effect on spore yield, biomass production, and efficacy, whereas the effect was not significant at low aeration. Specific spore and biomass yields also increased significantly with increasing glucose concentrations at high aeration. The scale of submerged fermentation was increased to 20-L fermentors, with dO levels of 10%, 30%, and 60% maintained by agitation and airflow controls. Further study will be required to optimize spore yields at the large scale.
This study led to development of a protocol for production of <i>C. truncatum</i> spores using submerged fermentation. Inoculum produced with this method can be used for laboratory, greenhouse, and field trials in development of the bioherbicide.
| Identifer | oai:union.ndltd.org:LACETR/oai:collectionscanada.gc.ca:SSU.etd-05312007-203817 |
| Date | 07 June 2007 |
| Creators | Dokken, Faye Louise |
| Contributors | Peng, Gary, Nelson, Louise M. |
| Publisher | University of Saskatchewan |
| Source Sets | Library and Archives Canada ETDs Repository / Centre d'archives des thèses électroniques de Bibliothèque et Archives Canada |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://library.usask.ca/theses/available/etd-05312007-203817/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to University of Saskatchewan or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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