The usefulness of detecting genomic instability in the form of microsatellite instability (MSI) has been examined in a number of cancers. Squamous cell carcinoma (SCC) and adjacent normal and lichen sclerosis (LS) samples were taken from patients with radical vulvectomies. Vulvar SCC samples were compared with adjacent normal samples using four oligonucleotide markers (BAT-25, BAT-26, D10S208 and D10S587) found to be informative with other carcinomas. Using polymerase chain reaction techniques, 30 vulvar SCC DNA samples were examined for MSI. BAT-26 displayed the highest level of MSI and was considered to be the most sensitive marker in studying vulvar SCC.
There was not a statistically significant difference between adjacent normal and LS samples analyzed. The level of MSI discovered in three loci (BAT-26, D10S208 and D10S587) was statistically significant when compared to adjacent normal samples and indicates a dysfunction in mismatch repair genes. Genomic instability pathways of carcinogenesis, characterized by mismatch repair defects and MSI, appear to have a role in the genesis of vulvar SCC, based on the high incidence of MSI in association with BAT-26.
| Identifer | oai:union.ndltd.org:LSU/oai:etd.lsu.edu:etd-04012004-144102 |
| Date | 08 April 2004 |
| Creators | Marlborough, Sidney Joseph |
| Contributors | Vince Wilson, Ralph Portier, Albert Cunningham |
| Publisher | LSU |
| Source Sets | Louisiana State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lsu.edu/docs/available/etd-04012004-144102/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to LSU or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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