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The distribution, diversity and functional characterization of the Listeria Genomic Island 1

Listeria monocytogenes was the causative agent of the nationwide 2008 outbreak associated with contaminated ready-to-eat meat products. Within the whole
genome DNA sequences of the outbreak isolates we previously identified a novel 50kb genomic island, designated as Listeria Genomic Island 1 (LGI1). LGI1 is predicted to
contribute to Listeria pathogenesis and/or environmental persistence because it encodes
genes related to known virulence factors and mobilization functions, including a putative
type IV secretion system and a putative small multidrug resistance efflux pump. The
distribution of LGI1 in Canadian L. monocytogenes isolates was determined by PCR
screening for LGI1 within 126 isolates from 1987 to 2010 that represented different
serotypes and pulsed-field gel electrophoresis (PFGE) patterns. To assess the evolutionary history and genetic diversity of this island, total LGI1 sequences from 15 whole-genome sequences were compared, and from the full study panel of isolates,PCR screening for the chromosomal insertion site and multiple LGI coding sequences were performed. LGI1 was detected almost exclusively in serotype 1/2a isolates, and within those, the isolates predominantly had the same PFGE patterns. These LGI1-encoding isolates also exclusively belonged to the multi-locus sequence typing (MLST)clonal complex 8. LGI1 was highly genetically conserved and it was inserted at the same location within the genome in 65 of the 67 isolates that harboured the island. To study the function and expression of LGI1, antimicrobial susceptibility assays,bioinformatic analyses and real-time reverse-transcription PCR were used. Isolates encoding LGI1 had an increased tolerance to quaternary ammonium compounds commonly used in sanitizing agents (benzalkonium chloride (BCl) and benzethonium
chloride (BeCl)) compared to isolates lacking LGI1 (but still highly related by MLST and PFGE). LGI1 is also tightly regulated, with expression of 13 of 16 tested coding sequences only being induced by the presence of sub-inhibitory concentrations of BCl,and one predicted regulator being expressed under all conditions. This study indicates
that the vast majority LGI encoding CC8 isolates share a common progenitor L.
monocytogenes ancestor that acquired LGI1 in a single evolutionary event. LGI1 has
remained genetically conserved since that time, and the functions contributed by this
island minimally include an increased tolerance to sanitizer agents.
Date10 January 2012
CreatorsZiegler, Jennifer
ContributorsGilmour, Matthew (Medical Microbiology), Wylie, John (Medical Microbiology) Van Domselaar, Gary (Pharmacy) Holley, Rick (Food Science)
Source SetsUniversity of Manitoba Canada
Detected LanguageEnglish

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