Phytoestrogens are naturally occurring chemicals of plant origin that have the ability to cause estrogenic and/or anti-estrogenic effects due to their structural similarities to the human hormone oestradiol. It has been proposed that phytoestrogens protect against a wide range of ailments, including breast and prostate cancers, cardiovascular disease, osteoporosis, and menopausal symptoms. Daidzein, biochanin A and especially genistein which has been reported to be the most biologically active dietary phytoestrogen attract great deal of interest in today&rsquo / s researches.
In this study, twenty different food items, including legumes, fruits, nuts and herbs, (haricot beans, chickpeas, green lentils, red lentils, soybeans, licorice root, yarrow, dried chestnuts, prunes, raisins, currants, black cumin, dried apricots, dried parsley, dried dates, dried figs, sage (from Aegean region), sage (from Mediterranean region), grapevine leaves, gilaburu) were selected. Following an extraction procedure employing acid hydrolysis and heating / they were analysed for their daidzein, genistein and biochanin A contents using a reversed-phase C18 column with linear gradient elution on a high-performance liquid chromatography (HPLC) coupled with diode-array detector (DAD).
Soybeans were found to contain high amounts of daidzein (91.36 mg/100 g) and genistein (85.57 mg/100 g). Chickpeas were found to contain much less amount of genistein (0.89 mg/100 g) compared with that of soybeans and also biochanin A (0.95 mg/100 g) which was not detected in soybeans. None of daidzein, genistein and biochanin A was detected in the remaining eighteen food items.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12605398/index.pdf |
| Date | 01 September 2004 |
| Creators | Gultekin, Esra |
| Contributors | Yildiz, Fatih |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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