ABSTRACT
DETERMINATION OF ARSENIC, SELENIUM AND CADMIUM IN SOME
TURKISH SPICES BY INDUCTIVELY COUPLED PLASMA MASS
SPECTROMETRY
Senol, Feriye
M.Sc., Department of Chemistry
Supervisor: Prof. Dr. O. Yavuz Ataman
July 2010, 106 Pages
The major source of all the nutrient elements for human body is the daily diet
which is affected by age, sex, and health status, geographic and climatic conditions.
It is important to analyze food items in order to determine their elemental contents,
estimate their dietary intakes and compare with recommended or tolerated intake
values in order to limit or increase their consumption. Spices are taken from different
parts of plants and widely used in cooking processes. They are convenient samples to
investigate the distribution of elements among different parts of plants and to
evaluate their daily intakes. Arsenic and selenium are both essential and toxic
elements whereas cadmium is considered to be toxic to human health. The
recommended dietary allowance (RDA) level of selenium ranges from 50 to 200
&mu / g/day. The RDA level of arsenic is set to be 100-200 &mu / g/day for adults to meet the
requirements. The weekly tolerable intake of cadmium and arsenic are 7 and 15
&mu / g/kg, respectively whereas selenium is toxic when intake is greater than 750 &mu / g/day
.
In this study the analysis of a variety of Anatolian spices including daisy
(Chamomillae Vulgaris), bay leaf (Folium Lauri), mint (Folium Menthane), rosehip
(Rosae Caninae), sage (Folium Salviae Officinalis), thyme (Herba Thymi), cumin
(Fructus Cummuni), sumac (Folium Rhois Coriariae), linden flower (Flos Tilliae)
v
and black pepper (Piper Nigrum) were performed and As, Cd and Se levels were
determined by using inductively coupled plasma mass spectrometry (ICP-MS). The
results obtained were evaluated together with the results of previous studies for
determination of Na, K, Mg, Ca, Li, Zn, Fe, Cu, B, Hg, Pb and Mn by inductively
coupled plasma optical emission spectrometry (ICPOES) and atomic absorption
spectrometry (AAS). Samples were digested in microwave oven in optimized
concentrations of HNO3 and H2O2. microwave temperature program was optimized
to maximize digestion efficiency. Samples were analyzed by using direct calibration
method for cadmium and standard addition method for arsenic and selenium
considering the effect of HNO3 concentration on ICP-MS signals. The accuracy of
the methods was checked by using Oyster Tissue 1566b SRM for cadmium and
arsenic and BCR Human Hair, 397 SRM for selenium. The mean arsenic and
selenium levels were found to be in the range of 100-500 &mu / g/ kg whereas cadmium
levels were relatively lower falling in a range of 10-100 &mu / g/ kg with few exceptions.
In order to investigate the effects of spectral and nonspectral interferences on arsenic
signals interference studies were performed by using HCl, NaCl, NaNO3, CsCl,
CsNO3, LiCl and LiNO3. Statistical evaluations were performed on data in order to
detect on significant trends.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12612225/index.pdf |
| Date | 01 July 2010 |
| Creators | Senol, Feriye |
| Contributors | Ataman, Osman Yavuz |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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