Salvia genus is a widely cultivated genus and used in medicine for various purposes as having
antimicrobial, antioxidant, anticarcinogen and anti-inflammatory features. In this study the aim
was to investigate phenolic composition of Salvia absconditiflora and understand the possible
effects of those constituents in cancer related drug metabolizing enzymes. Salvia absconditiflora
showed 80,43 % Radical Scavenging Activity against DPPH radical. Total flavonoid content was
found as one third of total phenolic content. Presence of important phenolic acids and flavonoids
such as caffeic acid, luteolin, coumaric acid are validated with LC-MS/MS analysis. Cytotoxicity of
Salvia absconditiflora treatment on MCF-7 and MDA-MB-231 breast cancer cell lines were
investigated through XTT and TBE assays both dose and time dependent manner. Cell
proliferation was inhibited 50 % by different IC50 values calculated in different assays and different
time intervals. This suggests that two breast cancer cell lines response in a different way to
cytotoxic treatments. Cancer related drug metabolizing enzyme gene modulations were
investigated with qRT-PCR. CYP1A1 and CYP1B1 were up-regulated in MCF-7 but down-regulated
in MDA-MB-231 cells in response to Salvia absconditiflora treatment.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12615623/index.pdf |
| Date | 01 January 2013 |
| Creators | Yilmaz, Selis |
| Contributors | Iscan, Mesude |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | Access forbidden for 1 year |
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