Aflatoxin is a highly carcinogenic secondary metabolite produced by Aspergillus flavus contributing to significant loss of corn production. There are several breeding techniques to make the corn resistant to aflatoxin accumulation with the genotype having desirable characters. Corn- resistant inbred line Mp313E provides resistance against A. flavus. Near-isogenic lines containing QTLs were developed by backcrossing Mp313E (resistant parent) and Va35 (susceptible parent). WRKY transcription factors play an important role in the growth and development of plants. Searching DNA markers associated with these WRKY genes is a considerable approach for the plant breeders. In this study, PCR and agarose gel electrophoresis methods were used to determine the polymorphic WRKY gene DNA markers associated with aflatoxin reduction quantitative trait loci in maize. The analysis revealed 26 polymorphic maize WRKY genes. Computational 3D models of these WRKY genes were predicted. Virtual 2D plot was made for these WRKY proteins to determine their position.
| Identifer | oai:union.ndltd.org:MSSTATE/oai:scholarsjunction.msstate.edu:td-6077 |
| Date | 30 April 2021 |
| Creators | Awasthi, Akanksha |
| Publisher | Scholars Junction |
| Source Sets | Mississippi State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | Theses and Dissertations |
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