The H-Ras protein is a GTPase important to cell cycle and differentiation. Mutations in this protein have been associated with 30% of cancers. A better understanding of this protein could lead to innovative treatments of cancers caused by the mutations. Solvent mapping of the crystallized Q61L mutant of the H-Ras protein with glycerol shows with potential areas of protein/protein interactions, areas that are of particular interest in the design of anti-cancer drugs. This study investigated whether glycerol can distinguish changes in the surface due to the mutation. A comparison was made between the wild-type and Q61L mutant H-Ras crystal structures in glycerol and aqueous solution. The structural analysis lead to the conclusion that the main changes observed were due to the solvent environment and not to the mutation.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-04062006-162655 |
| Date | 17 April 2006 |
| Creators | Holsenbeck, Stephanie Leah |
| Contributors | Carla Mattos, Linda Hanley-Bowdoin, Robert Rose |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-04062006-162655/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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