Genetic linkage maps have been constructed for many species of conifers; however, none has been previously published for an <i>Abies</i> species. A genetic linkage map of Nordmann fir (<i>Abies nordmanniana</i> (Steven) Spach) open-pollinated family 9M was constructed using AFLP and RAPD markers developed from megagametophytic DNA. In all, 556 markers were grouped at LOD ≥ 5.0, θ = 0.30, into 19 linkage groups that covered 1977 cM (Kosambi). Framework maps were ordered with interval support ≥ 3.0 for each linkage group. Accessory markers were attached to the nearest framework marker based on LOD scores and recombination fractions. Significant linkage distortion (approximately 10% of the 556 markers based on a chi-square test at p ≤ 0.05) from the expected 1:1 Mendelian segregation ratio was recognized. The genome size was estimated to be 2471 cM, and this map provided 80% coverage of the genome. Attempts to map a trait locus (based on disease phenotype in an inoculated mapping population) for disease resistance to <i>Phytophthora cinnamomi</i> Rands were unsuccessful. This <i>Abies</i> linkage map will be important to the Christmas tree industry for marker-assisted selection of useful traits such as pest resistance, branching characteristics, height, growth rate, and post-harvest needle retention.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-06142005-164308 |
| Date | 15 June 2005 |
| Creators | Hudson, Emily Elizabeth |
| Contributors | D. Michael Benson, Henry V. Amerson, L. John Frampton |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-06142005-164308/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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