The ability of bacteria to adapt to environmental stress is essential to their existence. Several responses to environmental triggers are known. Two of these, Competence and Sporulation, are widespread in the bacterial world. Competence and Sporulation are best studied in the organism Bacillus subtilis and the proteins involved in these pathways are well known. The purpose of this research is to gain insight into the regulation of these cellular processes by determining the NMR solution structures and looking at functional aspects of the ClpC R-domain, involved in bacterial competence, and the L66A mutant of the Spo0F protein involved in the regulation of sporulation. In addition to these studies attempts to purify a Spo0F homolog from the endospore-forming bacteria, Pasteuria penetrans, were made with the intention of determining the metal binding characteristics of the protein.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-11222006-142621 |
| Date | 21 December 2006 |
| Creators | McLaughlin, Patrick Daniel |
| Contributors | Dr. John Cavanagh, Dr. Mike Goshe, Dr. Dennis Brown, Dr. Charles Opperman, Dr. Ronald Venters |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-11222006-142621/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dis sertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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