Wounding stress is a continuous threat to the survival of all organisms, and, in crop plants it leads to a dramatic reduction in crop yield. The signaling pathways that allow plants to respond to wounding stress are known to be complex. Many plant hormones such as jasmonic acid (JA), salicylic acid (SA), and ethylene have been identified as critical factors for the induction of wound-responsive genes in a number of plant species. However, the specific roles of each hormone in vivo, and how different hormones interact in the wound-stress response are largely unknown. Elucidation of the specific interactions of these plant hormones in response to wound stress is a major focus of wound research. The major goal of this research is to utilize Arabidopsis JA, SA, and ethylene biosynthetic and signaling mutants to analyze the role of each hormone separately and together in wound-responsive gene induction. Arabidopsis wild type, ein2, npr1, jar1, npr1/ein2, and npr1/jar1 plants were wounded and sampled at different times, after which RNA was extracted, subjected to electophoresis, transferred to filters and probed for several putative wound-regulated transcripts, which included PDF1.2, PR1, WAK1, and, LOX2. The constitutive levels of transcript expression as well as patterns of accumulation of these transcripts in response to the wounding stimulus varied in the different mutants.
| Identifer | oai:union.ndltd.org:NCSU/oai:NCSU:etd-12302003-172335 |
| Date | 05 January 2004 |
| Creators | David, Lisa Illene |
| Contributors | Dr. Eric Davies, Dr. Jose Alonso, Dr. Rebecca Boston, Dr. George Allen |
| Publisher | NCSU |
| Source Sets | North Carolina State University |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://www.lib.ncsu.edu/theses/available/etd-12302003-172335/ |
| Rights | unrestricted, I hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to NC State University or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report. |
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