Abstract
The purpose of this study is to investigate the probability of Eimeria tenella as a carrier for the transmission of chicken infectious anemia virus¡]CIAV¡^. One-day-old specific pathogen free¡]SPF¡^chickens were inoculated with CIAV, and then infected 20 days later with E. tenella. CIAV proteins were detected by immunofluorescent test in schizont, gametocytes and zygotes of E. tenella. Oocysts were purified from infected chicken by NaNO3 gradient centrifugation. Oocyst DNA was extracted by the treatments of SDS, proteinase K, DNase and chloroform. PCR template preparated from oocyst was used for amplification of CIAV DNA which is a 420 bp DNA fragment. The sequence of the PCR product from oocyst is similar to that of CIAV DNA from blood of infected chicken. The oocysts of E. tenella containing CIAV could also be suggested by inducing cytopathic effect after inoculated into cultured MSB1 cells. Our results indicated aprobability that E. tenella oocysts contain CIAV proteins and nucleic acids and thus E. tenella might paly an important role in the epizootiology.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0621101-151521 |
| Date | 21 June 2001 |
| Creators | Huang, Wan-ting |
| Contributors | David Chao, Gan-Nan Chang, Yi-Yang Lien |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0621101-151521 |
| Rights | campus_withheld, Copyright information available at source archive |
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