Salvia miltiorrhiza (SM) preconditioning was reported to be helpful in the early
recovery of gastrointestinal motility in the intestinal congestion of rats with hepatic
ischemia reperfusion. The aim of this study was to determine whether SM stimulates
contraction of isolated terminal ileum of Sprague-Dawley rat ex vitro and the
mechanisms which regulates that. The roots of SM were extracted by ethanol. One of
the indicative marker of SM, Tanshinone IIA, was identified and quantified with high
performance liquid chromatography (HPLC), and the results showed that Tanshinone
IIA was 1190 £gg/ml in SM extract. The effects of contractile activity of SM extract at
various cumulative dosages on the rat isolated terminal ileum were studied in organ
bath. The area under curve above the baseline of contractile graphy of SM extract on
isolated terminal ileum was recorded. In order to explore the contractile mechanism of
SM extract on isolated terminal ileum, the individual pretreatment or use of atropine
(a muscarinic receptor antagonist), tetrodotoxin (a sodium channel blocker),
nifedipine (a calcium channel blocker), Ca2+ free Kreb¡¦s solution with EGTA, or
trifluoperazine (a calmodulin blocker) was given and then cumulative dosages (40 £gL,
100 £gL, 180 £gL, 280 £gL) of SM extract were added. In addition, we used Fura-2
pentakis acetoxymethyl ester to detect the change of intracellular calcium
concentration of intestinal epithelial cell-6 (IEC-6) induced in 1/1000-time or 1/10000-time dilution of SM extract. The result indicated SM extract significantly
simulated the contraction of isolated terminal ileum in a dose-dependent manner. The
individual addition or use of atropine, tetrodotoxin, nifedipine, or Ca2+ free Kreb¡¦s
solution with EGTA all could not down-regulate significantly the contraction of SM
extract on isolated terminal ileum. Trifluoperazine significantly down-regulated the
contraction of SM extract on isolated terminal ileum. In addiation, SM extract was
able to increase cytosolic calcium concentration of IEC-6 cells. In conclusion, the
mechanisms of contraction of SM extract on isolated terminal ileum of rat were
involved in calmodulin/Ca2+ associated contraction pathway.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0720111-182435 |
| Date | 20 July 2011 |
| Creators | Tsai, Ching-Chung |
| Contributors | Jong-Kang Liu, Jiin-Tsuey Cheng, Ping-Heng Tan, Li-Ching Chang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0720111-182435 |
| Rights | not_available, Copyright information available at source archive |
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