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Effects of Clematis Armandi extracts on permeability and short circuit current (Isc) across frog skin epithelium

Summary
Clmatis Armandi has been used frequently in traditional Chinese medicine for the treatment of diuretic symptoms. The mechanism of its action is unclear. Possible action of this substance may involve alternation of electrolyte transport through the epithelia membranes. In this study¡Atransepithelial conductance of frog skin was measured in vitro in voltage-clamped Ussing chambers. Adding Clematis Armandi extracts to apical surface induced a conductance increment of 1.21 £gS and an apical to serosal Isc of 28.78 £gA/cm2. The Isc can not be completely blocked by apical application of amiloride. Nifedipine and TEA had no effect on Clematis Armandi induced Isc decrease. These data indicate that frog skin is highly responsive to the concentrated Clematis Armandi extracts. The increase in Isc reflects changes in transepithelial transport of Na+ ions modulated at apical membrane. The enormous increase in transepithelial conductance suggests that in additional to enhancement of amiloride-sensitive Na+ channels, Clematis Armandi may also modulate other pathways, such as Cl- ion channel modulation, which needs further investigation.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0730102-095814
Date30 July 2002
CreatorsHan, Taishien
Contributorsnone, Jau-Cheng Liou, Chen-Fu Shaw
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730102-095814
Rightsunrestricted, Copyright information available at source archive

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