Mouse tumor susceptibility gene, tsg101, was discovered by Dr. Stanley Cohen at Stanford University in 1996. Subsequent studies have revealed multiple functions of tsg101. It participates in MDM2/p53 regulatory circuit, protein trafficking and regulation of cellular proliferation and differentiation. Knockout mouse experiment has demonstrated an essential role of tsg101 in early embryonic development, since tsg101 deficiency results in malformation of mesoderm, hence embryonic lethality. To further scrutinize the role of tsg101 in mouse tissues during the stages of organogenesis, we analyzed tsg101 protein steady state expression profile in the developing embryo. The data indicated that tsg101 was ubiquitously and diffusedly expressed in all early embryonic tissues, suggesting a role of tsg101 in rapid dividing early embryonic cells. Along the developmental path, tsg101 expressions became more specific in the epithelial cells of lung, intestine, kidney, thyroid, thymus and adrenal gland. This is concordant with previous report of tsg101 function in regulation of epithelial cell differentiation. In addition, tsg101 expression was found in pyramidal and stellate cells in cerebral cortex, substantia nigra cells of thalamus and Purkinje cells in cerebellum of adult mouse. Whether tsg101 participates in regulating the trafficking of neurotransmitters in these neuronal cells awaits further investigation. Surprisingly, we found congruous expression profile of thyroglobulin in these neuronal cells, except in stellate cells. Besides, the expression of TTF-1 was found in the VI layer of cerebral cortex and neuronal fiber in thalamus. Both thyroglobulin and TTF-1 are thyroid specific proteins, which are important for the development of brain tissues. Further investigation is urged to clarify whether these two proteins really present in these brain tissues and their functional role in these neuronal cells.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0730104-074157 |
| Date | 30 July 2004 |
| Creators | Chiang, Cheng-lun |
| Contributors | Jiin-Tsuey Cheng, Hung-Tu Huang, Ming-Hong Tai |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0730104-074157 |
| Rights | unrestricted, Copyright information available at source archive |
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