The thesis aims to study the motion of individual fluorescent molecules in the gel environments, and applies to the sensitive electrophoretic and dielectrophoretic studies. Firstly, dye molecules (Rhodamine B and DiI) are dispersed, and investigate the individual fluorescent spots. Later on, we study the motion of individual B-phycoerythrin molecules under external electrical field driving.
Due to the porosity, agarose gel is used to contain the liquid, and the dye molecules can freely move within the pores. Thus, one can easily observe the motion of individual dye molecules under high numerical aperture objectives. B-phycoerythrin is chosen for the high extinction coefficient, native charge, and good fluorescent properties. Our results indicate most dye molecules are attached in the rigid structure of the gel. Only very limited molecular motions are observed.
Moreover, we study the dielectrophoretic interaction of the dye molecules. Nano-electrodes are fabricated by electrolysis to have sub-micron aperture silver tips. Due to the high gradient of the electric field, it is used to have strong enough attractive forces around the apex of the tip, to overcome the thermal fluctuations. It allows us further trapping and manipulating small non-charged objects, up to single dye molecules.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0801107-210454 |
| Date | 01 August 2007 |
| Creators | Lee, Wei-lung |
| Contributors | Liuwen Chang, Jui-Hung Hsu, Che-Hsin Lin |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0801107-210454 |
| Rights | not_available, Copyright information available at source archive |
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