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Molecular phylogeny of Acetes using the sequences of mitochondrial 16S rDNA and cytochrome oxidase I Genes

Abstract
Acetes is a kind of macroholozooplankton. We can use the "genetic
analysis to know its evolution, zooplankton population structure and taxa.
The step is :ampified the 16S rDNA of the mitochondrial DNA and the
COI fragment DNA in Acetes, and then sequence. We get the genetic
distance between species of the 16S rDNA is 2.65~13.16%, and it's
1.43~25.42% of the COI fragment gene in Acetes. These results show that
the diversity of COI fragment DNA in Acetes is more than the 16S rDNA
of the mitochondria DNA. If we translated the COI gene DNA to amino
acid sequence, the genetic distance between species of the amino acid
sequence is about 1.83%~8.97%. By the way, we know that the genetic
distance between species of the amino acid sequences are clearly less
then the DNA sequences. Using the genetic data, we constructure a
phylogenetic tree. The tree divided the Acetes into two group : Japonicus
group and Erythraeus group, we can find the Acetes japonicus is the most
permitive species and the A. erythraeus is the last specation species in the
Acetes under the DNA sequences. But under the tree which was made
from the amino acid sequence, we found the Acetes erythraeus is the
most permitive species in the Acetes. The genetic distance between A.
intermedius and A. erythreaus were 5.44% and 18.01% of the 16S rDNA
and COI gene which indicated that the A. intermedius is true species. The
population of A. japonicus between Japon Sea and Taiwan showed 0.24%
and 2.36% of the 16S rDNA and COI gene that reveled two population
should have gene-flow. The populations diversity of the A. intermedius in
Taiwan showed the same way which had no different and the populations
in Taiwan should be a single population.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0807100-102753
Date07 August 2000
CreatorsPan, Tsung-Wei
Contributorsnone, none, none, none, none
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0807100-102753
Rightsnot_available, Copyright information available at source archive

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