The effect of natural compound AN30 on oral cancer cell line (Ca9-22), and normal oral cell line (HGF-1) was investigated. AN30 was purified from native fern plant Thelypteris torresiana. It had been reported to induce cell cycle arrest, apoptosis and DNA damage, cell growth inhibition in lung cancer cells, breast cancer cells and prostate cancer cells, but the effect in oral cancer is unknown. In a preliminary test, AN30 shows high toxicity to oral cancer cells but not normal oral cells. After treatment of AN30 to oral cancer cells, caspase activation and comet assays were performed to verity apoptosis induction. 2-D electrophoresis was used to find out the differentially expressed proteins between normal and cancer oral cell line under AN30 treatment. The proteins were further identified by LC/MS and western blot. The mechanism of these candidate proteins on drug toxicity remains further investigation.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0814112-153336 |
| Date | 14 August 2012 |
| Creators | Jhong, Rong-Chang |
| Contributors | Kuang_Hung Cheng, Hung-Wen Huang, Hsueh-Wei Chang |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0814112-153336 |
| Rights | user_define, Copyright information available at source archive |
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