This research is focus on the analysis of microbial diversity and existance of methanotrophic microbial strains in Wu-Shan-Ding mud volcano areas. The microbial distribution and diversity in soil surrounding the mud volcano were analyzed by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). The 16S rDNA sequences from PCR-DGGE bands were used to identify the bacterial strains with NCBI gene bank data. The results showed that the microbial diversity in the fresh erupted mud sample varied with other soil samples. The degrees of diversity were propotion to the distance away from the mud volcano. The main bacterial species found in the mud sample are Brevundimonas terrae, Ralstonia solanacearum, Ralstonia taiwanensis, Pseudonocardiaceae bacterium, Ochrobactrum anthropi, Burkholderia phytofirmans, Stenotrophmonas sp., Methylobacterium sp., Cryseobacterium sp., Sphingobacterium sp., Stenotrophomonas sp. MG-3, and Stenotrophomonas sp. EP01. According to documents, all of the above strains are tolerant to high salt and alkaline. The Ochrobactrum anthropi and Burkholderia phytofirmans were found only in the fresh erupted mud sample and the 5-meter soil sample, while Ralstonia mannitolilytica, Ralstonia pickettii, Amycolatopsis rugosa, and Maricaulis sp. were found only in 10-meter soil sample. In other word, all of the soil samples we examed in this study have their own specified bacterial strains. Two methanotrophic microganisms, Methylobacterium organophilum and Ochrobactrum anthropi were also successfully isolated in pure cultures. The biochemical characteristics of these two strains had been studied. These two strains show a potential might be able to use in the bioremediation of petroleum pollution.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0823107-174428 |
| Date | 23 August 2007 |
| Creators | Pao, Wei-han |
| Contributors | Jimmy Kao, Liu, Jong-Kang, Jimmy Kuo |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823107-174428 |
| Rights | off_campus_withheld, Copyright information available at source archive |
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