We have investigated the chemical constituents of the organic extracts of soft
coral Lobophytum crassum, collected at Dongsha Atoll. This study has led to the
isolation of eight new compounds, including six cembrane-type diterpenoids 1-6
and two a-tocopherol-type compounds 7 and 8 The chemical structures of
compounds 1-8 were elucidated by extensive analysis of 1D, 2D NMR
spectroscopic data (1H and 13C NMR¡B1H-1H COSY¡BHSQC¡BHMBC¡BNOESY),
UV, IR, CD and MS. Compounds 1 and 6 possess unprecedented diterpenoid
skeletons. The absolute configurations of compound 1 were determined using a
modified Mosher¡¦s method.
Compounds 1-8 were tested against A-549 (human lung epithelial
carcinoma)¡BHT-29 (human colon adenocarcinoma)¡BP-388 (mouse lymphocytic
leukemia) tumor cell lines. Compounds 1, 7, and 8 displayed modest cytotoxicity
against P-388 cell line with ED50 values of 3.2, 3.2, and 2.7 £gg/mL, respectively.
Compound 8 exhibited marginal cytotoxicity against H-29 cell line with an ED50
value of 3.9 £gg/mL.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0823110-120512 |
| Date | 23 August 2010 |
| Creators | Lin, Shih-tseng |
| Contributors | Ling-ching Chen, Chang-yih Duh, Ian-lih Tsai |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0823110-120512 |
| Rights | not_available, Copyright information available at source archive |
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