In order to search for bioactive compounds from the organic extracts of a Formosan soft coral Lemnalia flava fourteen natural compounds, including seven new nardosinane sesquiterpenoids flavalin B-H (1-7),
two new nornardosinane sesquiterpenoids flavalins I-J (8-9), along with five known compounds, 2-oxolemnacarnol (10), lemnacarnol (11), armatin F (12), (2R)-2-hydroxylemnal-1(10)-en-12-one (13) and laevinol B (14) were isolated from L. flava. The structures of these compounds were established by the detailed spectral analysis (IR, MS, 1D, 2D NMR)
and by comparison of the spectral data with those of the related known compounds. The structure of 1 was unambiguously proven by X-ray diffraction analysis. The absolute configuration of 13 was further
determined by a modified Mosher's method.
The neuroprotective effect compounds of 1¡V3 against the damage of 6-hydroxydopamine (6-OHDA) toward SH-SY5Y cells was also measured. The cytotoxicity of 6-OHDA on SH-SY5Y cells was significantly reduced by pretreatment of 1 at various concentrations. The
cytotoxicity of compounds 1¡V13 against human breast carcinoma (MCF-7), human colon carcinoma (WiDr), human laryngeal carcinoma (HEp 2), human medulloblastoma (Daoy) T-cell acute lymphoblastic
leukemia(CCRF-CEM), colon adenocarcinoma (DLD-1), human promyelocytic leukemia (HL-60) and murine leukemia (P388D1) cell lines was studied. The ability of 1¡V13 to inhibit the up-regulation of
pro-inflammatory iNOS (inducible nitric oxide synthase) and COX-2(cyclooxygenase-2) proteins in LPS (lipopolysaccharide)-stimulated RAW264.7 macrophage cells was examined, and it was
shown that no cytotoxic and anti-inflammatory activity could be found for these compounds.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0824110-180033 |
| Date | 24 August 2010 |
| Creators | Hung, Wen-Yu |
| Contributors | Fang-Rong Chang, Jyh-Horng Sheu, Ping-Jyun Sung, Yang-Chang Wu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0824110-180033 |
| Rights | not_available, Copyright information available at source archive |
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