Three thermophilic aerobic bacterial strain, Geobacillus thermoleovorans T4, Bacillus subtilis b5 and Bacillus licheniformis b6 were isolated from the wastewater of a Taiwan Sugar Company sugar refinery in Kaohsiung. All strains were capable of growth in cellulose containing medium. We found out that G. thermoleovorans T4 possessed both of the activity of cellulses and xylanase. This was the first report ever in this bacterial spices. Its endoglucanase was active at temperatures ranging from 30¢J to 100¢J, and pH ranging from 4.0 and 8.0. Its £]-glucosidase activities could be measured at 40¢J - 90¢J and pH 5.0 - 9.0. Its xylanase was active from 30¢J to 90¢J and pH from 3.0 to 10.0, while the exoglucanase was active at temperatures from 30¢J to 90¢J. The pH values of xylasnase was better than G. thermoleovorans K-3d. This enzyme was worth of possible in industry application. Both of B. subtilis b5 and B. licheniformis b6 contained a thermoactive endoglucanase. The endoglucanase of B. subtilis b5 was active at pH values between 4.0 and 7.5, and was thermostable at 100¢J for at least 1 hour ( retaining 60% of the original activity ). The stability of this enzyme was better than all known enzymes extracted from Bacillus spices. The endoglucanase of B. licheniformis b6 was active at pH values between 4.0 and 9.0, and thermostable at 60¢J for least 1 hour.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0903103-170513 |
| Date | 03 September 2003 |
| Creators | Yu, Chih-Li |
| Contributors | Jong-Kang Liu, Ssu-Ching Chin, C. M. Kao |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0903103-170513 |
| Rights | unrestricted, Copyright information available at source archive |
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