ROGDI is a novel gene which locates on human chromosome 16p13.3 and has unknown function. The length of ROGDI coding region is 864 bp which encodes 287 amino acids. According to our previous studies, overexpression of ROGDI increased proliferation in several human cell lines. CDK2 is a member of cyclin-dependent kinases (CDKs) which plays a key role in regulation of G1/S cell cycle transition and is activated during G1 and S phases. In this studies, we found that expression of CDK2 and cyclin A were up-regulated following overexpression of ROGDI in HEK293T. On the other hand, down-regulation of CDK2 and cyclin A was observed following silencing of ROGDI. In addition, the interaction between ROGDI and CDK2 was evidenced by using immunoprecipitation. Meanwhile, we observed co-localization of ROGDI and CDK2 in the nucleus. We also exploited a drug, olomoucine, known to selectively inhibit CDK2. The result demonstrated that ROGDI-enhanced cell proliferation was dependent on CDK2 activity. Flow cytometry profiles of depletion of ROGDI displayed G1/S phase arrest as well as up-reguated the expression of p53 and p21. Taken together, we suggest that ROGDI may be a positive regulator of cell cycle and its action is CDK2-depedent.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0906110-170249 |
| Date | 06 September 2010 |
| Creators | Huang, Cai-hua |
| Contributors | Hsu, Ching-Mei, Cheng, Jiin-Tsuey, Cho, Chung-Lung, HONG, YI-REN, Tai, Ming-Hong |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0906110-170249 |
| Rights | not_available, Copyright information available at source archive |
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