Typically chronic injury leads to hepatic fibrosis. No effective antifibrotic
drugs have been approved, but herbal drugs have potential on the therapy of
hepatic fibrosis. The objective of this study used TAA-induced liver fibrosis
mouse as a model to elucidate whether aqueous extract of the root of Pluchea
indica (PIAE) can reduce liver fibrosis triggered by TAA. Mice were
intraperitoneally injected with TAA (200 mg/Kg) three times per week as the
TAA group, and those of injected with PIAE once per week as the treatment
group. Three PIAE dosages of low- (0.5 mg/ml), medium- (1.0 mg/ml), and
high- (1.5 mg/ml) doses were applied. Control mice were intraperitoneally
injected with phosphate-buffered saline (2 ml/Kg) three times per week. Mice
were sacrificed after 4 or 8 week treatment. Mice serum glutamyl pyruvic
transaminases (GPT) were increased in the TAA group while the treatment
group effects were declined after 4 or 8 weeks. H&E, Reticular fiber, and Sirius
red staining revealed that TAA induced liver fibrosis and fibrotic lesions were
reduced by PIAE treatment. Hydroxyproline assay showed that TAA increased
collagen contents and PIAE significantly decreased collagen contents after 4 or
8 weeks. Collagen £\1 and £\-SMA mRNA levels were decreased after 4- or 8-
week PIAE treatments. The protein levels of ED2, £\-SMA, p53, and
phospho-p53 were all significantly declined on 4 or 8 weeks after PIAE
treatment. In conclusion, these results demonstrated that the aqueous extract of
P. indica shows anti-fibrotic effects on fibrogenesis of mouse liver.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0908109-170256 |
| Date | 08 September 2009 |
| Creators | Wu, Li-chuan |
| Contributors | Li-Jen Liao, Tse-Min Lee, Chung-Lung Cho, Ching-Mei Hsu |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | English |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0908109-170256 |
| Rights | off_campus_withheld, Copyright information available at source archive |
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